In vitro studies on gelatin/hydroxyapatite composite modified with osteoblast for bone bioengineering

Abstract

A promising route towards bone tissue engineering is made by the raw materials of the composite which mimics properties of the extracellular matrix components. Herein, the favourable human origin bone cells were seeded on the scaffold types to investigate the best co-culture system. The same has been achieved after the synthesis and characterization of gelatin/hydroxyapatite composites infused with chitosan for their calcium to phosphate (Ca/P)ratio by SEM-EDX. Also, in vitro biodegradation and bio-mineralization determined after immersing in lysozyme and SBF respectively. Uni-axial Compressive Strength (UCS), porosity, qualitative to quantitative phase development by XRD and FTIR were evaluated. The human bone cell-seeded composite was tested by flow cytometry, CLSM, SEM and DSC. This study statistically signified the human mesenchymal stem cell (hM)derived bone cell as potential raw material for minor to severe bone related tissue regenerative studies.